Developmental effects in zebrafish embryos (3 days post-fertilization) exposed to 10 µg/mL of crude extracts from Fischerella 52-1 (A); 1 (B); and 2 (C); shown for comparison is a vehicle (i.e., solvent) only control (D).

Teratogenicity of 1 tested at 5 µg/mL concentration following exposure for (a) 2 days post-fertilization (dpf); (b) 3 dpf; and (c) 5 dpf; Also shown are 5 dpf embryos with apparent partial recovery from teratogenic effects when embryos removed from treatment after 2 dpf (d); and 3 dpf (e).

Teratogenicity of 1 tested at 10 µg/mL concentration following exposure for (a) 2 days post-fertilization (dpf); (b) 3 dpf; and (c) 5 dpf; Also shown are 5 dpf embryos with apparent partial recovery from teratogenic effects when embryos removed from treatment after 2 dpf (d); and 3 dpf (e).

Teratogenicity of 1 tested at 5 µg/mL (1a–c) and 10 µg/mL (2a–c) in zebrafish embryos exposed until 5 dpf. For reference, an untreated control embryo at 5 dpf is shown (d); Notable effects on development at both concentrations include (a) overall lack of pigmentations, i.e., melanophores; (b) curvature of body axis; and (c) pericardial edema.

Teratogenicity of 2 tested at 10 µg/mL (a) in zebrafish embryos exposed until 5 dpf. For reference, an untreated control embryo at 5 dpf is shown (b).