FIGURE SUMMARY
Title

Optical stimulation of zebrafish hair cells expressing channelrhodopsin-2

Authors
Monesson-Olson, B.D., Browning-Kamins, J., Aziz-Bose, R., Kreines, F., Trapani, J.G.
Source
Full text @ PLoS One

The myo6b promoter drives ChR2-YFP expression in hair cells of the ear and lateral line.

(A) The myo6b:ChR2-YFP meganuclease construct was inserted into the zebrafish genome by plasmid injection along with the I-SceI enzyme into the 1-cell stage embryo. (B) Hair cells of a 5 days post fertilization (dpf) zebrafish larva express YFP (pseudo-colored yellow) in the lateral line and ear (arrow). The lateral line is composed of neuromasts, clusters of hair cells, at regular intervals around the head and the length of the fish (examples at arrowheads, scale bar 0.05 mm). B2 ChR2-YFP larval eye at 5 dpf. B3 Wild-type larval eye at 5 dpf. (C) YFP expression in neuromast hair cells. C2 DIC image of the neuromast C3 composite of YFP and DIC images (scale bar 0.0125 mm) (D) Adult transgenic fish continue to express ChR2-YFP (background removed for clarity, scale bar 2 mm). D2 YFP expression in an adult neuromast (scale bar 0.05 mm).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One