FIGURE SUMMARY
Title

Quantitative assay for TALEN activity at endogenous genomic loci

Authors
Hisano, Y., Ota, S., Arakawa, K., Muraki, M., Kono, N., Oshita, K., Sakuma, T., Tomita, M., Yamamoto, T., Okada, Y., and Kawahara, A.
Source
Full text @ Biol. Open
Fig. S3

Phenotypic analysis of an S1PR2-TALEN-injected embryo. (A) An uninjected embryo (28 hours post fertilization (hpf)): Tg(cmlc2:mRFP). All observed embryos had one heart (n=64 embryos), as visualised by mRFP expression driven by a cardiac-specific promoter, cmlc2 (cardiac myosin light chain 2). No cardia bifida (two-heart phenotype) was observed. (B) An S1PR2- TALEN-injected embryo (28 hpf). Half of the embryos (34/68), exhibited cardia bifida following the injection of S1PR2-TALEN mRNA (400 pg each) into the blastomere of 1- to 2-cell stage embryos. (C) An S1PR2-MO-injected embryo (28 hpf). Nearly half of the embryos (36/66) exhibited cardia bifida following S1PR2-MO (10 ng) injection, as described previously (Kawahara et al., 2009). All images are ventral views, with the anterior at the top. Scale bars: 200 μm.

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open
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