FIGURE SUMMARY
Title

ERK Signaling Regulates Light-Induced Gene Expression via D-Box Enhancers in a Differential, Wavelength-Dependent Manner

Authors
Mracek, P., Pagano, C., Fröhlich, N., Idda, M.L., Cuesta, I.H., Lopez-Olmeda, J.F., Sánchez-Vázquez, F.J., Vallone, D., and Foulkes, N.S.
Source
Full text @ PLoS One

Western blot analysis of phospho-ERK under blue and red light. Representative western blots of endogenous ERK (ERK), phospho-ERK (p-ERK) and Vinculin or α-Tubulin in PAC-2 cells during (A) 2 hours of either blue or red light exposure and (B) exposure for 36 hours to a 12 hours blue; 12 hours dark LD cycle. In panel A, the duration of light exposure of each sample is indicated in minutes (mins). These blots are representative of six independent experiments and the final quantification is presented graphically in Figure 3A. In panel B, the time points are indicated as zeitgeber times (ZT, where ZT0 represents lights on and ZT12 represents lights off). Note that the p-ERK and ERK antibodies both recognize the two forms of ERK (p42 and p44). Thus, with these antibodies two bands are more or less well resolved depending on the duration of electrophoresis. Levels of Vinculin or α-Tubulin were used as loading controls for each western blot. Blue and red bars above or below each blot represent the wavelengths and duration of light exposure while black bars indicate darkness. (C) Quantification of relative phospho-ERK levels from three independent experiments performed as in panel B. No significant circadian oscillation was observed as tested by Cosinor analysis (COSINOR v3.0.2 software, Antoni Diez-Noguera, University of Barcelona).

Acknowledgments
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