Breves et al., 2013 - Prolactin regulates transcription of the ion uptake Na+/Cl- cotransporter (ncc) gene in zebrafish gill. Molecular and Cellular Endocrinology   369(1-2):98-106 Full text @ Mol. Cell. Endocrinol.

Fig. 4 Effects of incubation time and oPRL on ncc (A), nhe3b (B), ecac (C), prlra (D), prlrb (E) and β-actin (F) gene expression in cultured gill filaments. Solid line, control; dashed line, oPRL (1.0 μg/ml). Gene expression is presented as fold-change from 0 h. Means ± SEM (= 6). Filaments were dissected from anesthetized fish and pre-incubated for 3 h prior to treatment with oPRL-supplemented medium. Effects of hormone treatment and time were evaluated by two-way ANOVA. For clarity, only the results of follow-up analyses for a significant main effect of hormone treatment are presented. *, **, ***Significantly different from the time-matched controls at < 0.05, 0.01 and 0.001, respectively, by Student’s t-test. Whole-mount in situ hybridization showing ncc expression in gill filaments cultured for 4 h in the presence or absence of oPRL as compared with pre-incubated and 0 h control filaments (G). Scale bar = 50 μm.

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ZFIN wishes to thank the journal Molecular and Cellular Endocrinology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and Cellular Endocrinology, 369(1-2), Breves, J.P., Serizier, S.B., Goffin, V., McCormick, S.D., and Karlstrom, R.O., Prolactin regulates transcription of the ion uptake Na+/Cl- cotransporter (ncc) gene in zebrafish gill, 98-106, Copyright (2013) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.