FIGURE SUMMARY
Title

Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons

Authors
Shakes, L.A., Abe, G., Eltayeb, M.A., Wolf, H.M., Kawakami, K., and Chatterjee, P.K.
Source
Full text @ BMC Genomics
Fig. 5

"Excision assay" performed with genomic DNA isolated from 2-day old zebrafish embryos: GFP expression in the embryos injected with iTol2-BACs were observed between 28 and 48 hours post fertilization (hpf) (upper panels). APPb:EGFP BAC 13 (panel 5A), APPb:EGFP BAC 2 (panel 5B), fgf24:EGFP BAC 37 (panel 5C) and fgf24:EGFP BAC 35 (panel 5D) display green fluorescence. The white arrowheads indicate the expected tissue-specific expressions of GFP in these embryos. The iTol2-BAC injected embryos were analyzed using the "excision assay" (lower panels) [16,4].
Fig. 6

EGFP fluorescence in transgenic F1 zebrafish: Panel A: Germline expression of EGFP fluorescence in zebrafish embryos with DNA from APPb:EGFP BAC clone 15. Panel B shows the germline expression of EGFP fluorescence with DNA from APPb:EGFP BAC 27, and Panel C shows germline expression from fgf24:EGFP BAC clone 37.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Genomics
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