ZSSJ and ZF cell lines express dicer1, ago1, and ago2. (A) Schematic showing the intron (line) and exon (box) organization of zebrafish dicer1, ago1, and ago2 and the location of primers (arrows) used for RT-PCR analysis. Primers were designed to amplify exons 6 and 9 of dicer1; 1 and 6 of ago1 and 1 and 4 of ago2. (B) RT-PCR analysis of dicer1, ago1, and ago2 expression in adult zebrafish tissues and (C) ZSSJ and ZF cell cultures. β-actin was used as a positive control and vasa as a gonad-specific control.

miRNA transfection efficiency of ZF and ZSSJ cells. ZF and ZSSJ cell cultures were transfected with siRNA coupled with a red fluorophore in a 12-well plate. Cell fluorescence was observed under a Nikon fluorescence microscope equipped with a digital camera. The pictures were taken 48 hpt.