- Title
-
Genomic organization and embryonic expression of miR-430 in medaka (Oryzias latipes): insights into the post-transcriptional gene regulation in early development
- Authors
- Tani, S., Kusakabe, R., Naruse, K., Sakamoto, H., and Inoue, K.
- Source
- Full text @ Gene
TDRD7 3′UTR is targeted at miR-430 binding sites in medaka. (A) Schematic representation of the injected reporter mRNAs. (B) Imperfect base-pairing between miR-430 and the target sites in 3′UTR of zebrafish TDRD7 (Mishima et al., 2006). (C and D) 4-dpf embryos injected with mRNA of wild-type (C) or mutated (D) constructs of GFP:TDRD7 3′UTR at one-cell stage. Two large arrowheads indicate eyes. Wild-type 3′UTR restricts GFP expression in PGCs (C, arrow), whereas mRNA carrying mutated miR-430 binding sites is expressed in whole embryo (D). The inset in (C) shows the enlargement of the gonadal region. Small arrowheads in (D) outline the gonads. (C′ and D′) Control DsRed expression in the embryos shown in (C) and (D). (E) Two examples of predicted base-pairing between medaka miR-430a and the putative target sites in the 3′UTR of medaka TDRD7. |
Reprinted from Gene, 449(1-2), Tani, S., Kusakabe, R., Naruse, K., Sakamoto, H., and Inoue, K., Genomic organization and embryonic expression of miR-430 in medaka (Oryzias latipes): insights into the post-transcriptional gene regulation in early development, 41-49, Copyright (2010) with permission from Elsevier. Full text @ Gene