FIGURE SUMMARY
Title

Expression and water calcium dependence of calcium transporter isoforms in zebrafish gill mitochondrion-rich cells

Authors
Liao, B.K., Deng, A.N., Chen, S.C., Chou, M.Y., and Hwang, P.P.
Source
Full text @ BMC Genomics

Expression patterns of zebrafish plasma membrane Ca2+-ATPases (PMCAs) and Na+/Ca2+ exchangers (NCXs) in various tissues by RT-PCR analysis. The zebrafish beta-actin was used as internal control to evaluate the relative amounts of cDNAs. The expected amplicon sizes are shown on the right. 1Cell, cDNA from the 1-cell stage of zebrafish embryos.

Double fluorescence in situ hybridizations of zecac/zpmca2 (A-C) and zecac/zncx1b (D-F) in frozen sections of zebrafish gills. A and D, zecac mRNA; B, zpmca2 mRNA; C, merged image of A and B; E, zncx1b mRNA; F, merged image of D and E. Arrow indicates the colocalization of zecac/zpmca2 (A-C) and zecac/zncx1 (D-F), respectively. An asterisk (*) indicates the zpmca2 signal without zecac colocalization (A and B) and the zncx1b signal without zecac colocalization (D and E). Scale bar = 20 μm.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult

Triple fluorescence in situ hybridizations (ISH) and immunocytochemistry (ICC) for zecac mRNA/zpmca2 mRNA/Na+-K+-ATPase (A-E) and zecac mRNA/zncx1b mRNA/Na+-K+-ATPase (F-J) in frozen sections of zebrafish gills. A-E are the same section, and F-J are another section. A, zpmca2 mRNA; B and G, zecac mRNA; C and H, Na+-K+-ATPase (NKA); D and I, bright field (BF); E, merged image of A-D; J, merged image of F-I. Colocalization of the 3 transporter mRNAs and protein was found respectively in E and J. Scale bar = 20 μm.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Genomics