The processing of 670 BAC clones for cytogenetic mapping in the zebrafish genome by FISH. (A) BAC clone zK030G05 (labeled in green, denoted by green arrows) is observed to be syntenic with a previously mapped BAC clone probe, zK007C07, which localizes to the short arm (p arm) of LG chromosome 3, (labeled in orange, denoted by orange arrows). (B) One BAC clone, zK110G19 (labeled in red), was observed to have two signals on the same chromosome, LG chromosome 4. The primary signal is on 4p with a secondary signal in the heterochromatic region of 4q. LG chromosome 4 is denoted by red arrows. (C) Seventeen clones had signals on two non-homologous chromosomes. For example, zC211K08 (labeled in orange) localized to the p arm of LG chromosome 22 and to the q arm heterochromatic region of LG chromosome 4 (denoted by orange arrows). The near-telomeric marker for the q arm of LG chromosome 22, zC118M01, is labeled in green (denoted by green arrows) and the near-telomeric marker for the p arm of LG chromosome 4, zK030C13, is labeled in white (denoted by white arrows). (D) Five BAC clones were pan-centromeric, such as zK171K22 (labeled in orange). (E) Five BAC clones were peri-centromeric. For example, zK120I24 (labeled in orange) localized to the p arm and near the centromere of LG chromosome 7 (denoted by orange arrows), the p arm and near the centromere of an unknown chromosome, and near the centromere of multiple chromosomes. The near-telomeric marker for the q arm of LG chromosome 7, zC128L16, is labeled in green (denoted by green arrows).

A chromosome inversion variant observed between the Tu strain and the AB strain. (A) In metaphase preparations from Tu embryos, BAC clone zK167C09 (labeled in red) was observed to have only homozygous signals near the centromere of LG chromosome 3q. zK188H10, which consistently localized near the middle of LG chromosome 3p, is labeled in green. (B) Three hybridization patterns were observed for zK167C09 in metaphase preparations from AB embryos including homozygous signals near the centromere of LG chromosome 3q, homozygous signals located medially on LG chromosome 3p, and a heterozygous state. The heterozygous pattern is depicted in this image with zK167C09 labeled in red and zK188H10, which consistently hybridized medially on LG chromosome 3p, labeled in green. White arrows denote LG chromosome 3 in (A) and (B).

Identification of a near-telomeric marker for the long arm of LG chromosome 4. A large portion of the q arm of LG chromosome 4 consists of a heterochromatic region, which made finding a near-telomeric marker probe difficult for this chromosome arm. zC079A18 (labeled in orange) was established as a near-telomeric marker probe for the q arm of LG chromosome 4 (denoted by orange arrows). Also shown is the near-centromeric marker for LG chromosome 4, zC091G03, (labeled in white, denoted by white arrows) and BAC clone zC207E19, which localizes to the heterochromatic region of the long arm of LG chromosome 4 (labeled in green, denoted by green arrows).