Vallone et al., 2004 - E-box function in a period gene repressed by light. Proceedings of the National Academy of Sciences of the United States of America   101(12):4106-4111 Full text @ Proc. Natl. Acad. Sci. USA

Fig. 1 RNase protection assay analysis of zfper4 (410-nt protected fragment, amino acid positions 88–225) and β-actin (282-nt protected fragment) was performed with RNA harvested at the indicated zeitgeber times (ZT0 is lights-on, ZT12 is lights-off) or CT. (A) Whole brain from adult zebrafish maintained in LD. (B) Six-day-old larvae raised in LD. (C) PAC-2 cells were maintained in LD for 5 days. Cells were entrained in LD and harvested in DD (D)orinLL(E). (F) Peak and trough samples from the LD, DD, and LL sets of PAC-2 cells (C–E) assayed together to allow comparison of the relative expression levels. (G) Larvae raised for 6 days in DD and then exposed to light for the indicated times (hours; + Light). Control larvae remained in DD and were harvested in parallel. (H) Analysis of PAC-2 cells equivalent to G.

EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Day 6 to Adult
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA