ZFIN ID: ZDB-EXP-171103-13
Experiment Conditions Description: bacterial treatment by injection: Burkholderia cenocepacia, chemical ablation: macrophage, chemical treatment by environment: metronidazole
bacterial treatment by injection: Burkholderia cenocepacia
Name: bacterial treatment by injection
Definition: Bacterial treatment where the bacteria are injected into the fish.
Ontology: Zebrafish Environment Condition Ontology [ZECO:0000230]
Name: Burkholderia cenocepacia
Synonyms: Burkholderia cepacia genomovar III
Ontology: Taxonomy for ZECO [NCBITaxon:95486]
chemical ablation: macrophage
Name: chemical ablation
Definition: Experimental condition which uses a chemical to ablate an anatomical structure or cell.
Ontology: Zebrafish Environment Condition Ontology [ZECO:0000169]
Name: macrophage
Synonyms: histiocyte
Definition: A mononuclear phagocyte present in variety of tissues, typically differentiated from monocytes, capable of phagocytosing a variety of extracellular particulate material, including immune complexes, microorganisms, and dead cells.
Ontology: Anatomy Ontology [ZFA:0009141]
chemical treatment by environment: metronidazole
Name: chemical treatment by environment
Definition: Chemical treatment condition in which the chemical is introduced through the environment. For zebrafish this is the tank water.
Ontology: Zebrafish Environment Condition Ontology [ZECO:0000238]
Name: metronidazole
Synonyms: 1-(2-hydroxy-1-ethyl)-2-methyl-5-nitroimidazole, 1-(2-hydroxyethyl)-2-methyl-5-nitroimidazole, 1-(beta-ethylol)-2-methyl-5-nitro-3-azapyrrole, 1-(beta-hydroxyethyl)-2-methyl-5-nitroimidazole, 1-(beta-oxyethyl)-2-methyl-5-nitroimidazole, 2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethanol, 2-methyl-1-(2-hydroxyethyl)-5-nitroimidazole, 2-methyl-3-(2-hydroxyethyl)-4-nitroimidazole, 2-methyl-5-nitroimidazole-1-ethanol, metronidazol, metronidazole, metronidazolum
Definition: A member of the class of imidazoles substituted at C-1, -2 and -5 with 2-hydroxyethyl, nitro and methyl groups respectively. It has activity against anaerobic bacteria and protozoa, and has a radiosensitising effect on hypoxic tumour cells. It may be given by mouth in tablets, or as the benzoate in an oral suspension. The hydrochloride salt can be used in intravenous infusions. Metronidazole is a prodrug and is selective for anaerobic bacteria due to their ability to intracellularly reduce the nitro group of metronidazole to give nitroso-containing intermediates. These can covalently bind to DNA, disrupting its helical structure, inducing DNA strand breaks and inhibiting bacterial nucleic acid synthesis, ultimately resulting in bacterial cell death.
Ontology: ChEBI [CHEBI:6909]  (EBI)
Publication: Mesureur et al., 2017