PUBLICATION

Zebrafish semaphorin Z1a collapses specific growth cones and alters their pathway in vivo

Authors
Shoji, W., Yee, C.S., and Kuwada, J.Y.
ID
ZDB-PUB-980519-1
Date
1998
Source
Development (Cambridge, England)   125: 1275-1283 (Journal)
Registered Authors
Kuwada, John, Shoji, Wataru
Keywords
Semaphorin; zebrafish; growth cone guidance; lateral line; cell signalling
MeSH Terms
  • Animals
  • Axons/drug effects
  • Axons/metabolism
  • Ganglia/cytology
  • Ganglia/growth & development*
  • Gene Expression Regulation, Developmental/genetics*
  • Glycoproteins/genetics
  • Glycoproteins/physiology*
  • Immunohistochemistry
  • In Situ Hybridization
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/physiology*
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology*
  • Recombinant Proteins/genetics
  • Recombinant Proteins/pharmacology
  • Semaphorin-3A
  • Zebrafish/embryology*
PubMed
9477326 Full text @ Development
Abstract
The semaphorin/collapsin gene family encodes secreted and transmembrane proteins several of which can repulse growth cones. Although the in vitro activity of Semaphorin III/D/Collapsin 1 is clear, recent analyses of two different strains of semaphorin III/D/collapsin 1 knockout mice have generated conflicting findings. In order to clarify the in vivo action of this molecule, we analyzed sema Z1a, a zebrafish homolog of semaphorin III/D/collapsin 1. The expression pattern of sema Z1a suggested that it delimited the pathway of the growth cones of a specific set of sensory neurons, the posterior ganglion of the lateral line, in zebrafish. To examine the in vivo action of this molecule, we analyzed (1) the pathways followed by lateral line growth cones in mutants in which the expression of sema Z1a is altered in an interesting way, (2) response of lateral line growth cones to exogenous Sema Z1a in living embryos, and (3) the pathway followed by lateral line growth cones when Sema Z1a is misexpressed by cells along their normal route. The results suggest that a repulsive action of Sema Z1a helps guide the growth cones of the lateral line along their normal pathway.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping