PUBLICATION

Isolation and characterization of a carbonic anhydrase homologue from the zebrafish (Danio rerio)

Authors
Peterson, R.E., Tu, C.K., and Linser, P.J.
ID
ZDB-PUB-970507-64
Date
1997
Source
Journal of molecular evolution   44(4): 432-439 (Journal)
Registered Authors
Linser, Paul J., Peterson, Robert (Chuck)
Keywords
carbonic anhydrase; zebrafish; Danio rerio; retina; molecular evolution; teleost; cyprinid
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carbonic Anhydrases/genetics*
  • Carbonic Anhydrases/isolation & purification
  • Carbonic Anhydrases/metabolism
  • Cloning, Molecular
  • DNA Primers
  • Evolution, Molecular
  • Genetic Variation
  • Isoenzymes/genetics
  • Isoenzymes/metabolism
  • Kinetics
  • Molecular Sequence Data
  • Phylogeny*
  • Zebrafish/genetics*
PubMed
9089083 Full text @ J. Mol. Evol.
Abstract
We have isolated a 29,000-Da carbonic anhydrase (CA) protein from the zebrafish, Danio rerio, sequenced two peptide fragments, and tentatively identified it as a high-activity CA by inhibition kinetics. We have also characterized a 1,537-bp message whose deduced sequence of 260 amino acids matches that of the isolated protein. This CA is clearly an alpha-CA based on the similarity of its sequence to that of other members of the alpha-CA gene family. A phylogenetic analysis suggested CAH-Z diverged after the branching of the CA-V and CA-VII genes and prior to the duplications that generated the CA-I, CA-II, and CA-III genes of amniotes. This marks the first characterization of the mRNA and its protein product from the CA gene of a teleost.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping