PUBLICATION

The zebrafish homeobox gene Hox-2.2 transcription unit potential regulatory regions and in situ localization of transcripts

Authors
Njolstad, P.R., Molven, A., Apold, J., and Fjose, A.
ID
ZDB-PUB-961014-835
Date
1990
Source
The EMBO journal   9: 515-524 (Journal)
Registered Authors
Fjose, Anders, Molven, Anders
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Cyprinidae/genetics*
  • Embryo, Nonmammalian/cytology
  • Genes, Homeobox*
  • Genes, Regulator*
  • Mice
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Sequence Homology, Nucleic Acid
  • Terminology as Topic
  • Transcription, Genetic*
  • Zebrafish/genetics*
PubMed
1968004 Full text @ EMBO J.
Abstract
The data presented in this report strongly suggest that the genome of the zebrafish, Brachydanio rerio, has a homeobox cluster which is equivalent to the murine Hox-2 locus. In support of this conclusion, we have found two closely linked zebrafish genes which are true homologues of the mouse Hox-2.1 and Hox-2.2 genes. Here we describe structural and functional properties of the zebrafish Hox-2.2 homologue hox-2.2. Furthermore, we have identified another zebrafish gene related to hox-2.2 which appears to correspond to the Hox-6.1 gene of the murine Hox-3 locus. In order to characterize the zebrafish hox-2.2 gene we have determined the genomic DNA sequence of a 3.4 kb SalI fragment. This revealed that the hox-2.2 transcription unit encodes a putative protein of 228 amino acids. The homeodomains of the murine Hox-2.2 and the zebrafish hox-2.2 proteins are almost identical and extensive sequence identity exists in other regions of the two proteins, which share 160 (70%) of the amino acid residues. Also in terms of expression, strong similarities were observed relative to the murine Hox-2.2 gene. Transcripts derived from zebrafish hox-2.2 start to accumulate when somite formation is initiated. Later in development these transcripts are detected mainly in the central nervous system. Reminiscent of Hox-2.2, the rostral boundary of zebrafish hox-2.2 expression is located in the posterior region of the hindbrain. Notably, untranslated regions of the hox-2.2 gene contain several short sequences closely related to a known homeodomain recognition sequence.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping