PUBLICATION
The zebrafish homeobox gene Hox-2.2 transcription unit potential regulatory regions and in situ localization of transcripts
- Authors
- Njolstad, P.R., Molven, A., Apold, J., and Fjose, A.
- ID
- ZDB-PUB-961014-835
- Date
- 1990
- Source
- The EMBO journal 9: 515-524 (Journal)
- Registered Authors
- Fjose, Anders, Molven, Anders
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Cyprinidae/genetics*
- Embryo, Nonmammalian/cytology
- Genes, Homeobox*
- Genes, Regulator*
- Mice
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Sequence Homology, Nucleic Acid
- Terminology as Topic
- Transcription, Genetic*
- Zebrafish/genetics*
- PubMed
- 1968004 Full text @ EMBO J.
Citation
Njolstad, P.R., Molven, A., Apold, J., and Fjose, A. (1990) The zebrafish homeobox gene Hox-2.2 transcription unit potential regulatory regions and in situ localization of transcripts. The EMBO journal. 9:515-524.
Abstract
The data presented in this report strongly suggest that the genome of the zebrafish, Brachydanio rerio, has a homeobox cluster which is equivalent to the murine Hox-2 locus. In support of this conclusion, we have found two closely linked zebrafish genes which are true homologues of the mouse Hox-2.1 and Hox-2.2 genes. Here we describe structural and functional properties of the zebrafish Hox-2.2 homologue hox-2.2. Furthermore, we have identified another zebrafish gene related to hox-2.2 which appears to correspond to the Hox-6.1 gene of the murine Hox-3 locus. In order to characterize the zebrafish hox-2.2 gene we have determined the genomic DNA sequence of a 3.4 kb SalI fragment. This revealed that the hox-2.2 transcription unit encodes a putative protein of 228 amino acids. The homeodomains of the murine Hox-2.2 and the zebrafish hox-2.2 proteins are almost identical and extensive sequence identity exists in other regions of the two proteins, which share 160 (70%) of the amino acid residues. Also in terms of expression, strong similarities were observed relative to the murine Hox-2.2 gene. Transcripts derived from zebrafish hox-2.2 start to accumulate when somite formation is initiated. Later in development these transcripts are detected mainly in the central nervous system. Reminiscent of Hox-2.2, the rostral boundary of zebrafish hox-2.2 expression is located in the posterior region of the hindbrain. Notably, untranslated regions of the hox-2.2 gene contain several short sequences closely related to a known homeodomain recognition sequence.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping