PUBLICATION
hsp 90 alpha and hsp 90 beta genes are present in the zebrafish and are differentially regulated in developing embryos
- Authors
- Krone, P.H. and Sass, J.B.
- ID
- ZDB-PUB-961014-639
- Date
- 1994
- Source
- Biochemical and Biophysical Research Communications 204: 746-752 (Journal)
- Registered Authors
- Krone, Patrick H., Sass, Jennifer
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA Primers
- Gene Expression Regulation, Developmental*
- HSP90 Heat-Shock Proteins/genetics*
- Molecular Sequence Data
- Multigene Family
- Sequence Homology, Amino Acid
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 7980538 Full text @ Biochem. Biophys. Res. Commun.
Citation
Krone, P.H. and Sass, J.B. (1994) hsp 90 alpha and hsp 90 beta genes are present in the zebrafish and are differentially regulated in developing embryos. Biochemical and Biophysical Research Communications. 204:746-752.
Abstract
We have employed a polymerase chain reaction-based cloning strategy to demonstrate that both hsp 90 alpha and hsp 90 beta genes are present in the zebrafish. The fact that zebrafish represents the most primitive vertebrate in which hsp 90 genes have been isolated to date has allowed us to determine that the duplication event which generated the hsp 90 alpha and hsp 90 beta genes occurred shortly before the emergence of the teleosts from the rest of the vertebrate lineage. In expression studies using Northern blot analysis, hsp 90 beta mRNA was found to be present at control temperatures throughout normal embryonic development whereas hsp 90 alpha mRNA was barely detectable. Upon heat shock, hsp 90 alpha mRNA levels increased dramatically in all developmental stages examined. The levels of hsp 90 beta mRNA increased 2-3 fold during heat shock of early stage embryos. Thus, the hsp 90 alpha gene is strongly upregulated during heat shock in zebrafish embryos whereas expression of the hsp 90 beta gene appears to be weakly induced.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping