PUBLICATION

Zebrafish pou[c]: a divergent POU family gene ubiquitously expressed during embryogenesis

Authors
Johansen, T., Moens, U., Holm, T., Fjose, A., and Krauss, S.
ID
ZDB-PUB-961014-492
Date
1993
Source
Nucleic acids research   21: 475-483 (Journal)
Registered Authors
Fjose, Anders, Johansen, Terje, Krauss, Stefan
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Blotting, Northern
  • DNA
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/metabolism
  • Embryo, Nonmammalian/metabolism
  • Humans
  • Molecular Sequence Data
  • Multigene Family*
  • Nucleic Acid Conformation
  • POU Domain Factors
  • Sequence Homology, Amino Acid
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed
8441661 Full text @ Nucleic Acids Res.
Abstract
We report the isolation and characterization of cDNA for a novel zebrafish (Brachyodanio rerio) POU domain gene, pou[c], which is ubiquitously expressed during embryonic development. This gene encodes a 610 amino acids long protein with a 149 amino acid POU domain ending only 8 residues before the C terminus. The 453 amino acids long region N-terminal to the POU domain contains several features typical of transcriptional activation domains such as an acidic region with a putative amphipathic alpha-helix, a glutamine-rich region, and short threonine- and/or serine-rich regions. Comparison of the POU domain of pou[c] to other known POU sequences clearly show that pou[c] has the most divergent POU domain sequence reported to date. Thus, we suggest that pou[c] should be placed as the presently sole member of a new, sixth class of POU proteins. DNA-binding studies revealed that pou[c] is not an octamer-binding transcription factor like the Oct proteins described from mammals, chicken and Xenopus. Rather, pou[c] binds with high affinity to the TAATGARAT motif found in the promoters of the herpes simplex virus immediate early genes and to degenerate octamer-TAATGA motifs. Circular permutation analyses also show that pou[c] induces DNA bending upon sequence-specific binding.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping