PUBLICATION
Suspended solids-associated toxicity of hydraulic fracturing flowback and produced water on early life stages of zebrafish (Danio rerio)
- Authors
- Lu, Y., Zhang, Y., Zhong, C., Martin, J.W., Alessi, D.S., Goss, G.G., Ren, Y., He, Y.
- ID
- ZDB-PUB-210629-11
- Date
- 2021
- Source
- Environmental pollution (Barking, Essex : 1987) 287: 117614 (Journal)
- Registered Authors
- Goss, Greg
- Keywords
- Flowback and produced water (FPW), Hydraulic fracturing (HF), Polycyclic aromatic hydrocarbons (PAHs), Suspended solids (SS), Toxicity, Zebrafish embryo
- MeSH Terms
-
- Animals
- Ecosystem
- Hydraulic Fracking*
- Water
- Water Pollutants, Chemical*/toxicity
- Zebrafish
- PubMed
- 34171731 Full text @ Environ. Pollut.
Citation
Lu, Y., Zhang, Y., Zhong, C., Martin, J.W., Alessi, D.S., Goss, G.G., Ren, Y., He, Y. (2021) Suspended solids-associated toxicity of hydraulic fracturing flowback and produced water on early life stages of zebrafish (Danio rerio). Environmental pollution (Barking, Essex : 1987). 287:117614.
Abstract
Hydraulic fracturing flowback and produced water (HF-FPW), which contains polyaromatic hydrocarbons (PAHs) and numerous other potential contaminants, is a complex wastewater produced during the recovery of tight hydrocarbon resources. Previous studies on HF-FPW have demonstrated various toxicological responses of aquatic organisms as consequences of combined exposure to high salinity, dissolved organic compounds and particle/suspended solids-bound pollutants. Noteworthy is the lack of studies illustrating the potentially toxic effects of the FPW suspended solids (FPW-SS). In this study, we investigated the acute and sublethal toxicity of suspended solids filtered from six authentic FPW sample collected from two fracturing wells, using a sediment contact assay based on early-life stages of zebrafish (Danio rerio). PAHs profiles and acute toxicity tests provided initial information on the toxic potency of the six samples. Upon exposure to sediment mixture at two selected doses (1.6 and 3.1 mg/mL), results showed adverse effects in larval zebrafish, as revealed by increased Ethoxyresorufin-O-deethylase (EROD) activity. Transcriptional alterations were also observed in xenobiotic biotransformation (ahr, pxr, cyp1a, cyp1b1, cyp1c1, cyp1c2, cyp3a65, udpgt1a1, udpgt5g1), antioxidant response (sod1, sod2, gpx1a, gpx1b) and hormone receptor signaling (esr1, esr2a, cyp19a1a, vtg1) genes. The results demonstrated that even separated from the complex aqueous FPW mixture, FPW-SS can induce toxicological responses in aquatic organisms' early life stages. Since FPW-SS could sediment to the bottom of natural wetland acting as a continuous source of contaminants, the current findings imply the likelihood of long-term environmental risks of polluted sediments on aquatic ecosystems due to FPW spills.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping