PUBLICATION

Regenerated interneurons integrate into locomotor circuitry following spinal cord injury

Authors
Vasudevan, D., Liu, Y.C., Barrios, J.P., Wheeler, M.K., Douglass, A.D., Dorsky, R.I.
ID
ZDB-PUB-210507-20
Date
2021
Source
Experimental neurology   342: 113737 (Journal)
Registered Authors
Barrios, Joshua, Dorsky, Richard, Vasudevan, Deeptha
Keywords
Neurogenesis, Regeneration, Spinal cord injury, Spinal interneurons, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Interneurons/physiology*
  • Locomotion/physiology*
  • Nerve Net/physiology*
  • Nerve Regeneration/physiology*
  • Neuronal Plasticity/physiology
  • Recovery of Function/physiology*
  • Spinal Cord Injuries/genetics
  • Spinal Cord Injuries/physiopathology*
  • Zebrafish
PubMed
33957107 Full text @ Exp. Neurol.
Abstract
Whereas humans and other adult mammals lack the ability to regain locomotor function after spinal cord injury, zebrafish are able to recover swimming behavior even after complete spinal cord transection. We have previously shown that zebrafish larvae regenerate lost spinal cord neurons within 9 days post-injury (dpi), but it is unknown whether these neurons are physiologically active or integrate into functional circuitry. Here we show that genetically defined premotor interneurons are regenerated in injured spinal cord segments as functional recovery begins. Further, we show that these newly-generated interneurons receive excitatory input and fire synchronously with motor output by 9 dpi. Taken together, our data indicate that regenerative neurogenesis in the zebrafish spinal cord produces interneurons with the ability to integrate into existing locomotor circuitry.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping