PUBLICATION

First Record of Clinostomum sp. (Digenea: Clinostomidae) in Danio rerio (Actinopterygii: Cyprinidae) and the Implication of Using Zebrafish from Pet Stores on Research

Authors
Silveira, T., Kütter, M.T., Martins, C.M.G., Marins, L.F., Boyle, R.T., Campos, V.F., Remião, M.H.
ID
ZDB-PUB-210304-12
Date
2021
Source
Zebrafish   18(2): 139-148 (Journal)
Registered Authors
Marins, Luis Fernando
Keywords
laboratory animal, metacercaria, parasite, pet store, yellow grub
MeSH Terms
  • Animals
  • Fish Diseases*/epidemiology
  • Fish Diseases*/parasitology
  • Metacercariae
  • Trematoda*
  • Trematode Infections*/epidemiology
  • Zebrafish/parasitology*
PubMed
33656385 Full text @ Zebrafish
Abstract
Many scientific studies still use zebrafish from pet stores as animal models, even cutting-edge researches. However, these animals differ genotypically and phenotypically between them. The importance of the use of standardized models is widely recognized. Besides that, another consequence of using zebrafish from unknown origins is the acquisition of parasitized animals. This study aimed to relate the infection by Clinostomum sp. in zebrafish. Animals sold as "high standard" were acquired from a commercial company. Swimming alterations and superficial yellow dots were observed in five zebrafish with clinical signs, which were isolated, euthanized, and necropsied. Muscular yellow cysts with metacercaria associated with lesions were observed. The muscular cysts were responsible for the superficial yellow dots as well as the swimming alterations. The prevalence was 2.5%, and the mean infection intensity was 7 digeneans/host. The cysts measured a mean of 1251.43 μm long × 784.28 μm wide. Metacercariae measured a mean of 4847 μm long × 1353 μm wide. This first report about infection by Clinostomum sp. in zebrafish is globally relevant since the host and the parasite genus currently overlap worldwide. Furthermore, this study sheds light on the importance of the specific pathogen-free commercial creations or laboratory-reared zebrafish for research.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping