PUBLICATION

Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining

Authors
Rostam, N., Dosch, R.
ID
ZDB-PUB-210220-39
Date
2021
Source
Methods in molecular biology (Clifton, N.J.)   2218: 245-252 (Chapter)
Registered Authors
Dosch, Roland
Keywords
Bucky ball, Fixation method, Germ plasm, Glyoxal, Immunostaining, PFA, Zebrafish
MeSH Terms
  • Animals
  • Antibodies/metabolism
  • Embryo, Mammalian/metabolism*
  • Female
  • Fixatives/metabolism
  • Glyoxal/metabolism*
  • Immunohistochemistry/methods*
  • Male
  • Staining and Labeling/methods
  • Tissue Fixation/methods*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism
PubMed
33606236 Full text @ Meth. Mol. Biol.
Abstract
Immunohistochemistry has been widely used as a robust technique to determine the cellular and subcellular localization of proteins. This information ultimately helps to understand the function of these proteins and how biological processes are regulated. Antibodies applicable for labeling in zebrafish are limited, making immuno-staining challenging. Recently glyoxal fixation was rediscovered in tissue culture, mouse, rat, and Drosophila, expanding the list of effective antibodies for these species. Here, we compare a protocol for zebrafish staining using glyoxal as a fixative agent with PFA. We demonstrate that glyoxal fixation improves the antigenicity of some epitopes thereby increasing the number of useful antibodies in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping