PUBLICATION
Cellular and Molecular Characterization of the Effects of the Zebrafish Embryo Genotyper Protocol
- Authors
- Douek, A.M., Klein, E.I., Kaslin, J., Currie, P.D., Ruparelia, A.A.
- ID
- ZDB-PUB-210123-26
- Date
- 2021
- Source
- Zebrafish 18(1): 92-95 (Journal)
- Registered Authors
- Currie, Peter D., Douek, Alon M., Kaslin, Jan, Ruparelia, Avnika
- Keywords
- ZEG, zebrafish embryo genotyper, zebrafish genotyping
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian*
- Genotyping Techniques/methods*
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 33481695 Full text @ Zebrafish
Citation
Douek, A.M., Klein, E.I., Kaslin, J., Currie, P.D., Ruparelia, A.A. (2021) Cellular and Molecular Characterization of the Effects of the Zebrafish Embryo Genotyper Protocol. Zebrafish. 18(1):92-95.
Abstract
The Zebrafish Embryo Genotyper (ZEG) device provides a promising tool for genotyping live embryos. Although the gross morphology and survival of embryos after the use of ZEG are unaffected, the cellular and molecular effects of the ZEG protocol remain unknown. To address this, we have examined the integrity of specific tissues, and evaluated the expression of stress-responsive genes to determine the impact of the ZEG protocol. Our analyses reveal that although ZEG results in a low-level acute stress response, no long-lasting effects are evident, supporting its utilization for a variety of downstream assays.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping