PUBLICATION

The Last Half Century of Fish Explant and Organ Culture

Authors
LeClair, E.E.
ID
ZDB-PUB-210120-21
Date
2021
Source
Zebrafish   18(1): 1-19 (Review)
Registered Authors
LeClair, Elizabeth E.
Keywords
cell culture, explant, media, organ culture, organoid, slice culture, tissue culture
MeSH Terms
  • Animals
  • Organ Culture Techniques/instrumentation
  • Organ Culture Techniques/methods*
  • Organ Culture Techniques/statistics & numerical data
  • Zebrafish*
PubMed
33464995 Full text @ Zebrafish
Abstract
Explants are three-dimensional tissue fragments maintained outside the organism. The goals of this article are to review the history of fish explant culture and discuss applications of this technique that may assist the modern zebrafish laboratory. Because most zebrafish workers do not have a background in tissue culture, the key variables of this method are deliberately explained in a general way. This is followed by a review of fish-specific explantation approaches, including presurgical husbandry, aseptic dissection technique, choice of media and additives, incubation conditions, viability assays, and imaging studies. Relevant articles since 1970 are organized in a table grouped by organ system. From these, I highlight several recent studies using explant culture to study physiological and embryological processes in teleosts, including circadian rhythms, hormonal regulation, and cardiac development.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping