PUBLICATION

A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes

Authors
Chen, X., Xu, J., Suo, F., Yu, C., Zhang, D., Chen, J., Wu, Q., Jing, S., Li, L., Huang, W.
ID
ZDB-PUB-201028-11
Date
2020
Source
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy   229: 117949 (Journal)
Registered Authors
Huang, Wei
Keywords
High-throughput, Imaging, Paper-based devices, Point-of-care testing, Zinc ions
MeSH Terms
  • Animals
  • Cattle
  • Fluoresceins/chemistry*
  • Fluorescent Dyes/chemistry*
  • HeLa Cells
  • Hep G2 Cells
  • Humans
  • Point-of-Care Testing/statistics & numerical data*
  • Serum/metabolism*
  • Spectrometry, Fluorescence
  • Water/metabolism*
  • Zebrafish/metabolism*
  • Zinc/analysis*
PubMed
31864152 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Abstract
The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn2+. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn2+ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn2+ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn2+ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn2+ in the living cells and zebrafishes.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping