PUBLICATION

Apical Resection and Cryoinjury of Neonatal Mouse Heart

Authors
Shen, H., Darehzereshki, A., Sucov, H.M., Lien, C.L.
ID
ZDB-PUB-200829-9
Date
2021
Source
Methods in molecular biology (Clifton, N.J.)   2158: 23-32 (Chapter)
Registered Authors
Lien, Ching-Ling (Ellen), Sucov, Henry M.
Keywords
Cardiomyocyte proliferation, Cryoinjury, Neonatal mouse, Scar formation, Ventricular apical resection
MeSH Terms
  • Animals
  • Animals, Newborn
  • Cardiac Surgical Procedures/adverse effects*
  • Cell Proliferation
  • Cryosurgery/adverse effects*
  • Female
  • Heart/physiology*
  • Heart Injuries/etiology
  • Heart Injuries/pathology*
  • Heart Injuries/rehabilitation
  • Male
  • Mice
  • Regeneration*
  • Ventricular Remodeling*
PubMed
32857362 Full text @ Meth. Mol. Biol.
Abstract
Neonatal mouse hearts have a regenerative capacity similar to adult zebrafish. Different cardiac injury models have been established to investigate the regenerative capacity of neonatal mouse hearts, including ventricular amputation, cryoinjury, and ligation of a major coronary artery. While the ventricular resection model can be utilized to study how tissue forms and regenerates de novo, cryoinjury and coronary artery ligation are methods that might better mimic myocardial infarction by creating tissue damage and necrosis as opposed to the removal of healthy tissue in the ventricular amputation model. Here we describe methods of creating ventricular resection and cardiac cryoinjury in newborn mice.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping