PUBLICATION

Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish

Authors
Marques, I.J., Sanz-Morejón, A., Mercader, N.
ID
ZDB-PUB-200829-10
Date
2021
Source
Methods in molecular biology (Clifton, N.J.)   2158: 51-61 (Chapter)
Registered Authors
Marques, Ines, Mercader Huber, Nadia
Keywords
Cryoinjury, Fibrosis, Heart, Regeneration, Ventricle apex, Zebrafish
MeSH Terms
  • Animals
  • Cardiac Surgical Procedures/adverse effects*
  • Cell Proliferation
  • Cryosurgery/adverse effects*
  • Disease Models, Animal*
  • Heart/physiology*
  • Heart Injuries/etiology
  • Heart Injuries/pathology*
  • Heart Injuries/rehabilitation
  • Regeneration*
  • Ventricular Remodeling*
  • Zebrafish
PubMed
32857365 Full text @ Meth. Mol. Biol.
Abstract
Zebrafish have the capacity to regenerate most of its organs upon injury, including the heart. Due to its amenability for genetic manipulation, the zebrafish is an excellent model organism to study the cellular and molecular mechanisms promoting heart regeneration. Several cardiac injury models have been developed in zebrafish, including ventricular resection, genetic ablation, and ventricular cryoinjury. This chapter provides a detailed protocol of zebrafish ventricular cryoinjury and highlights factors and critical steps to be considered when performing this method.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping