PUBLICATION
Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish
- Authors
- Marques, I.J., Sanz-Morejón, A., Mercader, N.
- ID
- ZDB-PUB-200829-10
- Date
- 2021
- Source
- Methods in molecular biology (Clifton, N.J.) 2158: 51-61 (Chapter)
- Registered Authors
- Marques, Ines, Mercader Huber, Nadia
- Keywords
- Cryoinjury, Fibrosis, Heart, Regeneration, Ventricle apex, Zebrafish
- MeSH Terms
-
- Animals
- Cardiac Surgical Procedures/adverse effects*
- Cell Proliferation
- Cryosurgery/adverse effects*
- Disease Models, Animal*
- Heart/physiology*
- Heart Injuries/etiology
- Heart Injuries/pathology*
- Heart Injuries/rehabilitation
- Regeneration*
- Ventricular Remodeling*
- Zebrafish
- PubMed
- 32857365 Full text @ Meth. Mol. Biol.
Citation
Marques, I.J., Sanz-Morejón, A., Mercader, N. (2021) Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish. Methods in molecular biology (Clifton, N.J.). 2158:51-61.
Abstract
Zebrafish have the capacity to regenerate most of its organs upon injury, including the heart. Due to its amenability for genetic manipulation, the zebrafish is an excellent model organism to study the cellular and molecular mechanisms promoting heart regeneration. Several cardiac injury models have been developed in zebrafish, including ventricular resection, genetic ablation, and ventricular cryoinjury. This chapter provides a detailed protocol of zebrafish ventricular cryoinjury and highlights factors and critical steps to be considered when performing this method.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping