PUBLICATION

A novel benzothiadiazole-based and NIR-emissive fluorescent sensor for detection of Hg2+ and its application in living cell and zebrafish imaging

Authors
He, H., Meng, X., Deng, L., Sun, Q., Huang, X., Lan, N., Zhao, F.
ID
ZDB-PUB-200810-12
Date
2020
Source
Organic & biomolecular chemistry   18(32): 6357-6363 (Journal)
Registered Authors
Zhao, Feng
Keywords
none
MeSH Terms
  • Animals
  • Cell Line
  • Fluorescent Dyes/chemical synthesis
  • Fluorescent Dyes/chemistry*
  • Humans
  • Infrared Rays
  • Mercury/analysis*
  • Molecular Structure
  • Optical Imaging*
  • Thiadiazoles/chemical synthesis
  • Thiadiazoles/chemistry*
  • Zebrafish
PubMed
32760945 Full text @ Org. Biomol. Chem.
Abstract
The development of a novel near-infrared (NIR) probe for the detection of toxic Hg2+ in organisms with high selectivity and sensitivity is of great interest but remains a great challenge. Hence, in this work, a new NIR fluorescence enhanced sensor (TBBA), which contains a D-A structure as the NIR fluorophore and rhodanine-3-acetic acid as the receptor, has been developed for the detection of Hg2+ with high selectivity, sensitivity, low limit of detection (13.10 nM) and good binding constant (2.37 × 104 M-1). The mechanism of TBBA response to Hg2+ was further proved by 1H NMR titration, HRMS, and theoretical calculations. Furthermore, TBBA is applied as a fluorescent probe for imaging living cells and zebrafish, indicating that it can be potentially applied for Hg2+ sensing in both environmental and biology fields.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping