PUBLICATION
A novel benzothiadiazole-based and NIR-emissive fluorescent sensor for detection of Hg2+ and its application in living cell and zebrafish imaging
- Authors
- He, H., Meng, X., Deng, L., Sun, Q., Huang, X., Lan, N., Zhao, F.
- ID
- ZDB-PUB-200810-12
- Date
- 2020
- Source
- Organic & biomolecular chemistry 18(32): 6357-6363 (Journal)
- Registered Authors
- Zhao, Feng
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line
- Fluorescent Dyes/chemical synthesis
- Fluorescent Dyes/chemistry*
- Humans
- Infrared Rays
- Mercury/analysis*
- Molecular Structure
- Optical Imaging*
- Thiadiazoles/chemical synthesis
- Thiadiazoles/chemistry*
- Zebrafish
- PubMed
- 32760945 Full text @ Org. Biomol. Chem.
Citation
He, H., Meng, X., Deng, L., Sun, Q., Huang, X., Lan, N., Zhao, F. (2020) A novel benzothiadiazole-based and NIR-emissive fluorescent sensor for detection of Hg2+ and its application in living cell and zebrafish imaging. Organic & biomolecular chemistry. 18(32):6357-6363.
Abstract
The development of a novel near-infrared (NIR) probe for the detection of toxic Hg2+ in organisms with high selectivity and sensitivity is of great interest but remains a great challenge. Hence, in this work, a new NIR fluorescence enhanced sensor (TBBA), which contains a D-A structure as the NIR fluorophore and rhodanine-3-acetic acid as the receptor, has been developed for the detection of Hg2+ with high selectivity, sensitivity, low limit of detection (13.10 nM) and good binding constant (2.37 × 104 M-1). The mechanism of TBBA response to Hg2+ was further proved by 1H NMR titration, HRMS, and theoretical calculations. Furthermore, TBBA is applied as a fluorescent probe for imaging living cells and zebrafish, indicating that it can be potentially applied for Hg2+ sensing in both environmental and biology fields.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping