PUBLICATION

Rapid well-plate assays for motor and social behaviors in larval zebrafish

Authors
Shen, Q., Truong, L., Simonich, M.T., Huang, C., Tanguay, R.L., Dong, Q.
ID
ZDB-PUB-200520-17
Date
2020
Source
Behavioural brain research   391: 112625 (Journal)
Registered Authors
Tanguay, Robyn L.
Keywords
Behavioral paradigm, Neurobehavioral, Rapid throughput screening, Zebrafish
MeSH Terms
  • Animals
  • Automation, Laboratory/methods
  • Behavior, Animal/physiology*
  • Embryo, Nonmammalian
  • High-Throughput Screening Assays/methods*
  • Larva/metabolism
  • Motor Activity/drug effects*
  • Motor Activity/physiology
  • Phenotype
  • Social Behavior
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism
PubMed
32428631 Full text @ Behav. Brain Res.
Abstract
Behavior phenotypes are a powerful means of uncovering subtle xenobiotic chemical impacts on vertebrate nervous system development. Rodents manifest complex and informative behavior phenotypes but are generally not practical models in which to screen large numbers of chemicals. Zebrafish recapitulate much of the behavioral complexity of higher vertebrates, develop externally and are amenable to assay automation. Short duration automated assays can be leveraged to screen large numbers of chemicals or comprehensive dose-response for fewer chemicals. Here we describe a series of mostly automated assays including larval photomotor response, strobe light response, blue color avoidance, shoaling and mirror stimulus-response performed on the ZebraBox (ViewPoint Behavior Technologies) instrument platform. To explore the sensitivity and uniqueness of each assay endpoint, larval cohorts from 5 to 28 days post fertilization were acutely exposed to several chemicals broadly understood to impact different neuro-activities. We highlight the throughput advantages of using the same instrument platform for multiple assays and the ability of different assays to detect unique phenotypes among different chemicals.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping