PUBLICATION

Promotion effect of liver tumor progression in male kras transgenic zebrafish induced by tris (1, 3-dichloro-2-propyl) phosphate

Authors
Chen, S., Gong, Z., Letcher, R.J., Liu, C.
ID
ZDB-PUB-200129-19
Date
2020
Source
Ecotoxicology and environmental safety   191: 110220 (Journal)
Registered Authors
Gong, Zhiyuan
Keywords
Flame retardants, Inflammatory response, Liver tumor, TDCIPP, Transgenic zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Carcinoma, Hepatocellular/chemically induced*
  • Disease Models, Animal
  • Liver Neoplasms/chemically induced*
  • Male
  • Organophosphates/toxicity*
  • Proto-Oncogene Proteins p21(ras)/genetics*
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
PubMed
31991394 Full text @ Ecotoxicol. Environ. Saf.
Abstract
A previous study reported that exposure to tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) could promote the progression of hepatocellular carcinoma (HCC) in female HCC model zebrafish. Due to the existence of gender disparity in the development of HCC between females and males, whether the promotion effect of TDCIPP still exists in male HCC model zebrafish remains unclear. In this study, Tg(fabp10:rtTA2s-M2; TRE2:EGFP-krasG12V), referred as kras transgenic zebrafish which was shown to be an inducible liver tumor model, was applied as experimental model to assess the promotion potential of TDCIPP for HCC in males. In brief, kras males were exposed to 20 mg/L doxycycline (DOX), 0.3 mg/L TDCIPP and a binary mixture of 20 mg/L DOX with 0.3 mg/L TDCIPP, and after exposure liver size, histopathology and transcriptional profiles of liver from these treatments were examined. With the involvement of TDCIPP, the liver size was significantly increased and the lesion of hepatocyte became more aggressive. Furthermore, expressions of genes involved in DNA replication and inflammatory response were simultaneously up-regulated in the treatment of TDCIPP compared with the solvent control and in the treatment of the binary mixture of the two chemicals compared to the single DOX treatment. Overall, our results suggested that TDCIPP had promotion effect on the progression of liver tumor in kras males.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping