PUBLICATION

Lipids Fingerprinting of Different Materials Sources by UPLC-Q-Exactive Orbitrap/MS Approach and Their Zebrafish-based Activities Comparison

Authors
Li, X., Li, C., Zhu, Y., Shi, Y., Zhang, X., Zhang, S., Wang, L., Lin, H.W., Hou, H., Hsiao, C.D., Han, L., Liu, K.
ID
ZDB-PUB-200125-4
Date
2020
Source
Journal of Agricultural and Food Chemistry   68(7): 2007-2015 (Journal)
Registered Authors
Hsiao, Chung-Der
Keywords
none
MeSH Terms
  • Animals
  • Chromatography, High Pressure Liquid
  • Fatty Acids/chemistry
  • Fatty Acids/metabolism
  • Glycerophospholipids/chemistry
  • Glycerophospholipids/metabolism
  • Lipid Metabolism*
  • Lipids/chemistry*
  • Mass Spectrometry
  • Sphingolipids/chemistry
  • Sphingolipids/metabolism
  • Zebrafish/metabolism*
PubMed
31975591 Full text @ J. Agric. Food Chem.
Abstract
Lipids are considered to be critical contributors to the nutrition, structural function, metabolic function, and other biological roles. They vary from one biological source to another. Here, the lipids from three common sources (soybean, egg yolk, and shrimp head) were comprehensively compared and characterized using lipidomics approach, together with UPLC-Q-Exactive Orbitrap/MS method. A total of 3027 lipid structures containing 778 FA, 750 GL, 1283 GP, 200 SP, and 16 ST were firstly identified and quantified in these resources. The characteristic lipid species with significant difference among groups were determined by lipidomics analysis. Besides, the antithrombotic, antioxidant, and anti-inflammatory activities were evaluated based on the zebrafish model. The correlation between differential lipids and activities was also analyzed. Our comprehensive lipidomics profiling and bioactivities of lipids from different sources in vivo can provide evidence for their future applications.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping