PUBLICATION

In-situ Imaging of Cysteine in the Brains of Mice with Epilepsy by a Near-infrared Emissive Fluorescent Probe

Authors
Li, S., Song, D., Huang, W., Li, Z., Liu, Z.
ID
ZDB-PUB-200108-8
Date
2020
Source
Analytical chemistry   92(3): 2802-2808 (Journal)
Registered Authors
Liu, Zhihong, Li, Zhen
Keywords
none
MeSH Terms
  • Animals
  • Brain/diagnostic imaging*
  • Cyclic N-Oxides/chemical synthesis
  • Cyclic N-Oxides/chemistry*
  • Cysteine/analysis*
  • Epilepsy/diagnostic imaging*
  • Fluorescent Dyes/chemical synthesis
  • Fluorescent Dyes/chemistry*
  • Infrared Rays
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Molecular Structure
  • Optical Imaging
  • Organophosphorus Compounds/chemical synthesis
  • Organophosphorus Compounds/chemistry*
  • Oxidative Stress
  • PC12 Cells
  • Rats
  • Spectrometry, Fluorescence
  • Zebrafish
PubMed
31903746 Full text @ Anal. Chem.
Abstract
Epilepsy is characterized by oxidative stress in the brain. As the crucial reductive biothiol, cysteine (Cys) directly regulates the occurrence of oxidative stress in the brain. Observations suggest that the decreased cysteine in plasma could potentially serve as a redox biomarker in temporal lobe epilepsy. However, due to the complexity of the brain and lack of appropriate in-situ detecting tools, the concentration change and regulation of Cys in epileptic brains remain unclear. Here, we report a near-infrared imaging probe (named Mito-CP) for tracking endogenous Cys in brains of pentylenetetrazole (PTZ)-induced epileptic seizures with high sensitivity and selectivity. Using this probe, we achieved an in-situ visualization of the increased Cys in PC12 cells under dithiothreitol stimulation. In addition, Mito-CP was able to real-time monitor Cys fluctuation in lipopolysaccharide-mediated oxidative stress in zebrafish. Ultimately, we directly visualized the precipitous reduction of Cys in epileptic brains for the first time. Mito-CP also revealed the fluctuation of Cys in epileptic brains during the treatment by an antiepileptic drug, curcumin. This work provides an effective tool for Cys imaging in brains and will help to expand the understanding of the pathogenesis of epilepsy.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping