PUBLICATION
A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros
- Authors
- Kolatsi-Joannou, M., Osborn, D.
- ID
- ZDB-PUB-191110-5
- Date
- 2020
- Source
- Methods in molecular biology (Clifton, N.J.) 2067: 25-39 (Chapter)
- Registered Authors
- Osborn, Dan
- Keywords
- Diabetes, Glomerular filtration, Nephropathy, Proteinuria, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Dextrans/chemistry
- Dextrans/metabolism
- Diabetic Nephropathies/diagnosis
- Diabetic Nephropathies/genetics
- Diabetic Nephropathies/pathology
- Diabetic Nephropathies/urine
- Disease Models, Animal
- Embryo, Nonmammalian/physiology
- Female
- Fluorescent Dyes/chemistry
- Fluorescent Dyes/metabolism
- Genes, Reporter/genetics
- Glomerular Filtration Barrier/pathology*
- Glomerular Filtration Barrier/physiology
- Kidney Glomerulus/metabolism
- Kidney Glomerulus/pathology
- Male
- Pronephros/metabolism
- Pronephros/pathology*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 31701443 Full text @ Meth. Mol. Biol.
Citation
Kolatsi-Joannou, M., Osborn, D. (2020) A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros. Methods in molecular biology (Clifton, N.J.). 2067:25-39.
Abstract
With the advances in next-generation sequencing and rapid filtering of candidate variants in diseased patients, it has been increasingly important to develop translatable in vivo models to study genetic changes. This allows for functional validation of pathogenic mutations and establishes a system to understand the etiology of disease. Due to the ease of genetic manipulation and rapid ex utero development, the zebrafish has become a valuable resource to study important biological processes, including nephrogenesis. The development and function of the zebrafish pronephros are akin to that of mammals. As such, they offer a tractable model to study kidney disease, especially diabetic nephropathy. However, in order to study kidney dysfunction in zebrafish it is imperative that an appropriate readout is available. The appearance of macro-proteins in patient's urine is indicative of defective kidney function. In this technical chapter, we describe the in vivo use of fluorescently tagged dextrans of different molecular weights to reveal the integrity of the zebrafish glomerular filtration barrier.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping