PUBLICATION

In vivo analysis of renal epithelial cells in zebrafish

Authors
Li, Y., Xu, W., Jerman, S., Sun, Z.
ID
ZDB-PUB-190909-1
Date
2019
Source
Methods in cell biology   154: 163-181 (Chapter)
Registered Authors
Sun, Zhaoxia
Keywords
Apical-basal polarity, CRISPR, Cilia, Epithelial cells, Kidney, Zebrafish
MeSH Terms
  • Animals
  • Base Sequence
  • CRISPR-Associated Protein 9/genetics
  • CRISPR-Associated Protein 9/metabolism
  • CRISPR-Cas Systems
  • Cell Polarity
  • Cilia/metabolism
  • Cilia/ultrastructure
  • Embryo, Nonmammalian/anatomy & histology
  • Embryo, Nonmammalian/cytology*
  • Embryo, Nonmammalian/metabolism
  • Epithelial Cells/cytology*
  • Epithelial Cells/metabolism
  • Female
  • Gene Editing/methods*
  • Immunohistochemistry/methods*
  • Kidney/cytology*
  • Kidney/embryology
  • Kidney/metabolism
  • Male
  • Organogenesis/genetics
  • RNA, Guide, Kinetoplastida/genetics
  • RNA, Guide, Kinetoplastida/metabolism
  • Zebrafish
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics*
PubMed
31493817 Full text @ Meth. Cell. Biol.
Abstract
The zebrafish kidney has been used effectively for studying kidney development, repair and disease. New gene editing capability makes it a more versatile in vivo vertebrate model system to investigate renal epithelial cells in their native environment. In this chapter we focus on dissecting gene function in basic cellular biology of renal epithelial cells, including lumen formation and cell polarity, in intact zebrafish embryos.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping