PUBLICATION

Tris(1,3-dichloro-2-propyl)phosphate (TDCIPP) disrupts zebrafish tail fin development

Authors
Rhyu, D., Lee, H., Tanguay, R.L., Kim, K.T.
ID
ZDB-PUB-190811-2
Date
2019
Source
Ecotoxicology and environmental safety   182: 109449 (Journal)
Registered Authors
Tanguay, Robyn L.
Keywords
Developmental toxicity, OPFRs, TDCIPP, Tail malformation, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Embryonic Development/drug effects*
  • Flame Retardants/metabolism
  • Flame Retardants/toxicity*
  • Larva
  • Organophosphates/metabolism
  • Organophosphorus Compounds/toxicity*
  • Phosphates/metabolism
  • Zebrafish/embryology
  • Zebrafish/metabolism
PubMed
31398778 Full text @ Ecotoxicol. Environ. Saf.
CTD
31398778
Abstract
The flame retardant, tris(1,3-dichloro-2-propyl) phosphate (TDCIPP), is one of the most developmentally toxic organophosphate flame retardants (OPFRs). However, few mechanistic studies on phenotypic malformation caused by TDCIPP have been conducted. This study investigates the molecular mechanism underlying abnormal tail fin development consistently observed in zebrafish embryos exposed to TDCIPP. The results show that the defects in the tail fin (e.g., bent spine, defective caudal fin, and damaged tip) were associated with altered expression of transcription factors. The significant up-regulation of mmp9 and, among insulin-growth factor (IGF) families, igfbp-1a and igfbp1b was observed, whereas alterations in the expression of cdx4, igf1a, ifg1b, igf2b, and vegaa regulating tail development were dependent on time points. In accordance with changes in mRNA gene expression, TDCIPP impaired vessel formation and disorganized muscle in transgenic Tg(fli-GFP) zebrafish larvae. Furthermore, we found that the overexpression of mmp9 caused by TDCIPP was not linked to igfbp-1. Overall, these findings demonstrate that TDCIPP disrupts the progression of tail fin development, accompanied by defects in vessel and muscle formation in developing zebrafish embryos.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping