PUBLICATION

Silver nanoparticle exposure impairs ion regulation in zebrafish embryos

Authors
Lee, C.Y., Horng, J.L., Chen, P.Y., Lin, L.Y.
ID
ZDB-PUB-190804-3
Date
2019
Source
Aquatic toxicology (Amsterdam, Netherlands)   214: 105263 (Journal)
Registered Authors
Horng, Jiun-Lin
Keywords
Ion regulation, Ionocyte, Mitochondria, Rhodamine 123, SIET, Silver nanoparticles, Skin, Zebrafish
MeSH Terms
  • Acids/metabolism
  • Animals
  • Cell Count
  • Cell Shape/drug effects
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism*
  • Ions
  • Larva/drug effects
  • Metal Nanoparticles/toxicity*
  • Silver/toxicity*
  • Survival Analysis
  • Water Pollutants, Chemical/toxicity
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
PubMed
31376794 Full text @ Aquat. Toxicol.
Abstract
The potential toxicity of silver nanoparticles (AgNPs) to the early stages of fish is still unclear. This study used zebrafish embryos as a model to investigate the toxic effects of AgNPs on ion regulation by skin ionocytes. Zebrafish embryos were exposed to AgNPs for 96 h (4-100 h post-fertilization (hpf)) or 4 h (96-100 hpf). After 96 h of exposure to 5 and 10 mg/L AgNPs, survival rates had decreased to 42% and 0%, respectively; the body length had also significantly decreased at 5 mg/L. Whole-body Na+ and K+ contents significantly decreased at 1 and 3 mg/L, while Ca2+ contents decreased at ≥0.1 mg/L. H+ secretion by the skin significantly decreased at 1 mg/L. The density of skin ionocytes labeled with rhodamine 123 (a mitochondrion marker) decreased by 25% and 55% at 1 and 3 mg/L, respectively; and 54% of ionocytes (at 3 mg/L) were deformed from an oval to a spinous shape. After 4 h of exposure to 1 and 5 mg/L, whole-body Na+ and Ca2+ contents, H+ secretion, and density of ionocytes had also significantly decreased. This study revealed the toxicity of AgNPs to skin ionocytes and ion regulation in the early stages of zebrafish embryos.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping