PUBLICATION
Silver nanoparticle exposure impairs ion regulation in zebrafish embryos
- Authors
- Lee, C.Y., Horng, J.L., Chen, P.Y., Lin, L.Y.
- ID
- ZDB-PUB-190804-3
- Date
- 2019
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 214: 105263 (Journal)
- Registered Authors
- Horng, Jiun-Lin
- Keywords
- Ion regulation, Ionocyte, Mitochondria, Rhodamine 123, SIET, Silver nanoparticles, Skin, Zebrafish
- MeSH Terms
-
- Acids/metabolism
- Animals
- Cell Count
- Cell Shape/drug effects
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism*
- Ions
- Larva/drug effects
- Metal Nanoparticles/toxicity*
- Silver/toxicity*
- Survival Analysis
- Water Pollutants, Chemical/toxicity
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- PubMed
- 31376794 Full text @ Aquat. Toxicol.
Citation
Lee, C.Y., Horng, J.L., Chen, P.Y., Lin, L.Y. (2019) Silver nanoparticle exposure impairs ion regulation in zebrafish embryos. Aquatic toxicology (Amsterdam, Netherlands). 214:105263.
Abstract
The potential toxicity of silver nanoparticles (AgNPs) to the early stages of fish is still unclear. This study used zebrafish embryos as a model to investigate the toxic effects of AgNPs on ion regulation by skin ionocytes. Zebrafish embryos were exposed to AgNPs for 96 h (4-100 h post-fertilization (hpf)) or 4 h (96-100 hpf). After 96 h of exposure to 5 and 10 mg/L AgNPs, survival rates had decreased to 42% and 0%, respectively; the body length had also significantly decreased at 5 mg/L. Whole-body Na+ and K+ contents significantly decreased at 1 and 3 mg/L, while Ca2+ contents decreased at ≥0.1 mg/L. H+ secretion by the skin significantly decreased at 1 mg/L. The density of skin ionocytes labeled with rhodamine 123 (a mitochondrion marker) decreased by 25% and 55% at 1 and 3 mg/L, respectively; and 54% of ionocytes (at 3 mg/L) were deformed from an oval to a spinous shape. After 4 h of exposure to 1 and 5 mg/L, whole-body Na+ and Ca2+ contents, H+ secretion, and density of ionocytes had also significantly decreased. This study revealed the toxicity of AgNPs to skin ionocytes and ion regulation in the early stages of zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping