PUBLICATION

Neurodevelopmental toxicity assessments of alkyl phenanthrene and Dechlorane Plus co-exposure in zebrafish

Authors
Chen, X., Chen, Y., Huang, C., Dong, Q., Roper, C., Tanguay, R.L., Zhu, Y., Zhang, Y.
ID
ZDB-PUB-190604-10
Date
2019
Source
Ecotoxicology and environmental safety   180: 762-769 (Journal)
Registered Authors
Tanguay, Robyn L.
Keywords
Alkyl phenanthrene, Co-exposure, Dechlorane plus, Neurodevelopmental toxicity, Synergistic effect
MeSH Terms
  • Animals
  • Apoptosis Regulatory Proteins/genetics
  • Drug Synergism
  • Hydrocarbons, Chlorinated/toxicity*
  • Nervous System/drug effects*
  • Nervous System/growth & development
  • Phenanthrenes/chemical synthesis
  • Phenanthrenes/toxicity*
  • Polycyclic Compounds/toxicity*
  • RNA, Messenger/metabolism
  • Reactive Oxygen Species/metabolism
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/growth & development
  • Zebrafish/physiology*
PubMed
31154201 Full text @ Ecotoxicol. Environ. Saf.
Abstract
Alkyl phenanthrene (A-Phen) and Dechlorane Plus (DP) are ubiquitous environmental pollutants that widely co-exist in the environment. It has been established that both A-Phen and DP elicit neurotoxicity, but the potential interactive toxicity of these contaminants is not well-known. To determine whether a mixture of A-Phen and DP would exhibit interactive effects on neurodevelopment, we co-exposed 3-methylphenanthrene (3-MP), a representative of A-Phen, with DP. Our results illustrated that exposure to 5 or 20 μg/L 3-MP alone or in combination with 60 μg/L DP caused neurobehavioral anomalies in zebrafish. In accordance with the behavioral deficits, 3-MP alone or co-exposed with DP significantly decreased axonal growth of secondary motoneurons, altered intracellular Ca2+ homeostasis and induced cell apoptosis in the muscle of zebrafish. Additionally, 3-MP alone or co-exposed with DP significantly increased reactive oxygen species (ROS) and the mRNA levels of apoptosis-related genes. These findings indicate that 3-MP alone or co-exposed with DP induces neurobehavioral deficits through the combined effects on neuronal connectivity and muscle function. Chemical analysis revealed significant increases in 3-MP and DP bioaccumulation in zebrafish co-exposed with 3-MP and DP. Elevated bioaccumulation resulting from mixture exposure may represent a significant contribution of the synergistic effects observed in combined chemical exposure.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping