PUBLICATION

Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode

Authors
Xie, J., Jusuf, P.R., Goodbourn, P.T., Bui, B.V.
ID
ZDB-PUB-190416-14
Date
2019
Source
Journal of visualized experiments : JoVE   (145): (Journal)
Registered Authors
Jusuf, Patricia, Xie, Jiaheng
Keywords
none
MeSH Terms
  • Animals
  • Electrodes*
  • Electroretinography/methods*
  • Larva/physiology*
  • Retina/physiology*
  • Retinal Cone Photoreceptor Cells/physiology*
  • Vision, Ocular/physiology*
  • Zebrafish
PubMed
30985748 Full text @ J. Vis. Exp.
Abstract
The zebrafish (Danio rerio) is commonly used as a vertebrate model in developmental studies and is particularly suitable for visual neuroscience. For functional measurements of visual performance, electroretinography (ERG) is an ideal non-invasive method, which has been well established in higher vertebrate species. This approach is increasingly being used for examining the visual function in zebrafish, including during the early developmental larval stages. However, the most commonly used recording electrode for larval zebrafish ERG to date is the glass micropipette electrode, which requires specialized equipment for its manufacture, presenting a challenge for laboratories with limited resources. Here, we present a larval zebrafish ERG protocol using a cone-shaped sponge-tip electrode. The novel electrode is easier to manufacture and handle, more economical, and less likely to damage the larval eye than the glass micropipette. Like previously published ERG methods, the current protocol can assess outer retinal function through photoreceptor and bipolar cell responses, the a- and b-wave, respectively. The protocol can clearly illustrate the refinement of visual function throughout the early development of zebrafish larvae, supporting the utility, sensitivity, and reliability of the novel electrode. The simplified electrode is particularly useful when establishing a new ERG system or modifying existing small-animal ERG apparatus for zebrafish measurement, aiding researchers in the visual neurosciences to use the zebrafish model organism.
Genes / Markers
Figures
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping