PUBLICATION
Effect of MS222 on Hemostasis in Zebrafish
- Authors
- Deebani, A., Iyer, N., Raman, R., Jagadeeswaran, P.
- ID
- ZDB-PUB-190331-4
- Date
- 2019
- Source
- Journal of the American Association for Laboratory Animal Science : JAALAS 58(3): 390-396 (Journal)
- Registered Authors
- Jagadeeswaran, Pudur
- Keywords
- none
- MeSH Terms
-
- Aminobenzoates/pharmacology*
- Anesthesia
- Anesthetics/pharmacology*
- Animals
- Blood Cell Count/veterinary
- Blood Coagulation/drug effects
- Hemostasis/drug effects*
- Zebrafish/blood*
- PubMed
- 30925946 Full text @ J. Am. Assoc. Lab. Anim. Sci.
Citation
Deebani, A., Iyer, N., Raman, R., Jagadeeswaran, P. (2019) Effect of MS222 on Hemostasis in Zebrafish. Journal of the American Association for Laboratory Animal Science : JAALAS. 58(3):390-396.
Abstract
MS222 is a compound used in anesthetizing vertebrates, including fish and frogs. Several side effects of this anesthetichave been reported, but its effect on hemostasis has not been studied. In our laboratory, we have used zebrafish for morethan 2 decades as a model system to study hemostasis. During this period, we have had trouble in collecting blood from anesthetizedzebrafish and observed more rapid blood clotting than in nonanesthetized counterparts. However, no systematic studies regarding the effect of MS222 on zebrafish hemostasis are available. In this study, we performed various assays suchas gill bleeding, measurement of Hct, total blood cell counts, thrombocyte counts, thrombocyte aggregation, and coagulationand measured the amount of blood collected. We found that Hct values, the amount of blood collected, bleeding, andcoagulation differed significantly between anesthetized and nonanesthetized fish. Our results suggest that blood collectedafter MS222 anesthesia of zebrafish has altered hemostasis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping