PUBLICATION

Preparation of Ruthenium Complex-Functionalized Two-Photon-Excited Red Fluorescence Silicon Nanoparticles Composite for Target Fluorescence Imaging and Photodynamic Therapy in Vitro

Authors
Dou, Y.K., Shang, Y., He, X.W., Li, W.Y., Li, Y.H., Zhang, Y.
ID
ZDB-PUB-190323-9
Date
2019
Source
ACS applied materials & interfaces   11(15): 13954-13963 (Journal)
Registered Authors
Li, Yuhao
Keywords
none
MeSH Terms
  • Animals
  • Cell Survival/drug effects
  • Coordination Complexes/chemistry*
  • Coordination Complexes/pharmacology
  • Coordination Complexes/therapeutic use
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes
  • Folic Acid/chemistry
  • HeLa Cells
  • Humans
  • Mice
  • Mice, Nude
  • Microwaves
  • Nanoparticles/chemistry*
  • Nanoparticles/therapeutic use
  • Nanoparticles/toxicity
  • Neoplasms/drug therapy
  • Neoplasms/pathology
  • Optical Imaging
  • Photochemotherapy
  • Ruthenium/chemistry*
  • Silicon/chemistry*
  • Singlet Oxygen/metabolism
  • Toxicity Tests
  • Zebrafish
PubMed
30901518 Full text @ ACS Appl. Mater. Interfaces
Abstract
Silicon nanoparticles (SiNPs), especially those emitting red fluorescence, have been widely applied in the field of bioimaging. However, harsh synthetic conditions and strong biological autofluorescence caused by short wavelength excitation restrict the further development of SiNPs in the field of biological applications. Here, we reported a method for synthesizing ruthenium complex-functionalized two-photon-excited red fluorescence silicon nanoparticles composite (SiNPs-Ru) based on fluorescence resonance energy transfer (FRET) under mild experimental conditions. In the prepared SiNPs-Ru composite, silicon nanoparticles synthesized by atmospheric pressure microwave-assisted synthesis served as fluorescence energy donor which had two-photon fluorescence properties, and tris(4,4'-dicarboxylic acid-2,2-bipyridyl) ruthenium(II) dichloride (LRu) acted as fluorescence energy acceptor which could emit red fluorescence as well as had the ability to produce singlet-oxygen for photodynamic therapy. Therefore, the synthesized SiNPs-Ru could emit red fluorescence by two-photon excitation (TPE) based on fluorescence resonance energy transfer, which could effectively avoid the interference of biological autofluorescence. Fluorescence imaging tests in zebrafish and nude mice indicated that the as-prepared SiNPs-Ru could act as a new kind of fluorescence probe for fluorescence imaging in vivo. By coupling folic acid (FA) to SiNPs-Ru, the prepared composite (FA-SiNPs-Ru) could not only serve as targeted two-photon fluorescence imaging probe but also kill the cancer cells via photodynamic therapy (PDT) in vitro.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping