PUBLICATION

Intercellular Pathways from the Vasculature to the Forming Bone in the Zebrafish Larval Caudal Fin: Possible Role in Bone Formation

Authors
Akiva, A., Nelkenbaum, O., Schertel, A., Yaniv, K., Weiner, S., Addadi, L.
ID
ZDB-PUB-190313-1
Date
2019
Source
Journal of structural biology   206(2): 139-148 (Journal)
Registered Authors
Yaniv, Karina
Keywords
Biomineralization, cryo FIB SEM, forming bone, intercellular space
MeSH Terms
  • Animal Fins/physiology*
  • Animals
  • Animals, Genetically Modified
  • Blood Vessels/physiology*
  • Bone Development*
  • Coloring Agents/administration & dosage
  • Larva/metabolism*
  • Zebrafish/growth & development*
PubMed
30858049 Full text @ J. Struct. Biol.
Abstract
The pathway of ion supply from the source to the site of bone deposition in vertebrates is thought to involve transport through the vasculature, followed by ion concentration in osteoblasts. The cells deposit a precursor mineral phase in vesicles, which are then exocytosed into the extracellular matrix. We observed that the entire skeleton of zebrafish larvae, is labelled within minutes after injection of calcein or FITC-dextran into the blood. This raised the possibility that there is an additional pathway of solute transport that can account for the rapid labelling. We used cryo- FIB SEM serial block face imaging to reconstruct at high resolution the 3D ultrastructure of the caudal tail of the zebrafish larva. This reconstruction clearly shows that there is a continuous intercellular pathway from the artery to the forming bone, and from the forming bone to the vein. Fluorescence light microscopy shows that calcein and FITC-dextran form a reticulate network pattern in this tissue, which we attribute to the dye being present in the intercellular space. We conclude that this intercellular continuous space may be a supply route for ions, mineral and other solute or particulate material to the fast forming bone.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping