PUBLICATION

Anticonvulsant Activity of Pterostilbene in Zebrafish and Mouse Acute Seizure Tests

Authors
Nieoczym, D., Socała, K., Gawel, K., Esguerra, C.V., Wyska, E., Wlaź, P.
ID
ZDB-PUB-190129-7
Date
2019
Source
Neurochemical research   44(5): 1043-1055 (Journal)
Registered Authors
Esguerra, Camila V., Gawel, Kinga Aurelia
Keywords
Depression, Mice, Pterostilbene, Seizures, Side effects, Zebrafish
MeSH Terms
  • Animals
  • Anticonvulsants/therapeutic use*
  • Antidepressive Agents/pharmacology
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Electroshock/adverse effects
  • Mice
  • Muscle Strength/drug effects*
  • Pentylenetetrazole/pharmacology
  • Seizures/chemically induced
  • Seizures/drug therapy*
  • Stilbenes/pharmacology*
  • Zebrafish
PubMed
30689162 Full text @ Neurochem. Res.
Abstract
Pterostilbene (PTE), a natural dimethylated analog of resveratrol, possesses numerous health-beneficial properties. The ability of PTE to cross the blood-brain barrier raised the possibility that this compound may modulate central nervous system functions, including seizure activity. The aim of our study was to investigate the activity of PTE in the larval zebrafish pentylenetetrazole (PTZ) seizure assay and three acute seizure tests in mice, i.e., in the maximal electroshock seizure threshold (MEST), 6 Hz-induced psychomotor seizure threshold and intravenous (iv) PTZ tests. Additionally, potential antidepressant activity of PTE was estimated in the forced swim test in mice. The chimney test was used to determine the influence of PTE on motor coordination in mice, while its influence on neuromuscular strength was assessed in the grip strength test in mice. Locomotor activity was determined to verify the results from the forced swim test. PTE revealed an evident anticonvulsant effect both in zebrafish larvae (10 µM; 2 h-incubation) and mice (at doses of 100 and 200 mg/kg, intraperitoneally) but it did not exhibit antidepressant potential in the forced swim test. Furthermore, it did not cause any statistically significant changes in motor coordination, neuromuscular strength and locomotor activity in mice. In conclusion, our present findings demonstrate for the first time the anticonvulsant potential of PTE. The aforementioned results suggest that it might be employed in epilepsy treatment, however, further precise studies are required to verify its activity in other experimental seizure and epilepsy models and its precise mechanism of action should be determined.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping