PUBLICATION

Visualize Embryogenesis and Cell Fate Using Fluorescent Probes with Aggregation-Induced Emission

Authors
Hu, F., Manghnani, P.N., Kenry, ., Feng, G., Wu, W., Teh, C., Liu, B.
ID
ZDB-PUB-190119-4
Date
2019
Source
ACS applied materials & interfaces   11(4): 3737-3744 (Journal)
Registered Authors
Teh, Cathleen
Keywords
aggregation-induced emission, bright signals, cell entrapped, embryonic cell, small molecule, zebrafish lineage tracing
MeSH Terms
  • Animals
  • Cell Differentiation/physiology*
  • Cell Division/physiology
  • Dextrans/chemistry
  • Horseradish Peroxidase/metabolism
  • Zebrafish
PubMed
30656936 Full text @ ACS Appl. Mater. Interfaces
Abstract
Horseradish peroxidase (HRP) and fluorogen-dextran conjugate are tracers extensively used for injection-based lineage tracing. However, HRP is sensitive to proteolytic digestion, whereas the high-molecular-weight dextran may have antigenicity. Small molecular tracers can overcome these problems, but they usually diffuse from labeled cells, causing inaccurate information. Herein, we developed a small-molecular-weight fluorogen with aggregation-induced emission (AIEgen) for embryonic cell tracing with strong signals against tracer dilution caused by cell division. Once injected into the ancestor cells, the AIEgen can be entrapped in the cells without leakage because of the two hydrophilic and neutral arms. Consequently, it can specifically trace the progenies of the treated ancestor cells. More importantly, the operating concentration of AIEgen can be much higher than that of fluorogens with aggregation-caused quenching, which provides bright signals in daughter cells during embryonic cell tracing, thus overcoming the problem of fast signal degradation typically encountered with the use of traditional cell tracers.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping