PUBLICATION

A bacterial immunomodulatory protein with lipocalin-like domains facilitates host-bacteria mutualism in larval zebrafish

Authors
Rolig, A.S., Sweeney, E.G., Kaye, L.E., DeSantis, M.D., Perkins, A., Banse, A.V., Hamilton, M.K., Guillemin, K.
ID
ZDB-PUB-181107-6
Date
2018
Source
eLIFE   7: (Journal)
Registered Authors
Guillemin, Karen
Keywords
Aeromonas, immunology, infectious disease, inflammation, lipocalin, microbiology, microbiota, mutualism, zebrafish
MeSH Terms
  • Aeromonas/genetics*
  • Animals
  • Host-Pathogen Interactions/genetics*
  • Host-Pathogen Interactions/immunology
  • Immunity, Innate/genetics*
  • Intestines/immunology
  • Intestines/microbiology
  • Larva/immunology
  • Larva/microbiology
  • Lipocalins/genetics
  • Lipocalins/immunology
  • Protein Domains/genetics
  • Symbiosis/genetics*
  • Symbiosis/immunology
  • Zebrafish/immunology
  • Zebrafish/microbiology
PubMed
30398151 Full text @ Elife
Abstract
Stable mutualism between a host and its resident bacteria requires a moderated immune response to control bacterial population size without eliciting excessive inflammation that could harm both partners. Little is known about the specific molecular mechanisms utilized by bacterial mutualists to temper their hosts' responses and protect themselves from aggressive immune attack. Using a gnotobiotic larval zebrafish model, we identified an Aeromonas secreted immunomodulatory protein, AimA. AimA is required during colonization to prevent intestinal inflammation that simultaneously compromises both bacterial and host survival. Administration of exogenous AimA prevents excessive intestinal neutrophil accumulation and protects against septic shock in models of both bacterially and chemically induced intestinal inflammation. We determined the molecular structure of AimA, which revealed two related calycin-like domains with structural similarity to the mammalian immune modulatory protein, lipocalin-2. As a secreted bacterial protein required by both partners for optimal fitness, AimA is an exemplar bacterial mutualism factor.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping