PUBLICATION
Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury
- Authors
- Ratnayake, D., Currie, P.D.
- ID
- ZDB-PUB-181028-6
- Date
- 2019
- Source
- Methods in molecular biology (Clifton, N.J.) 1889: 245-254 (Other)
- Registered Authors
- Currie, Peter D., Ratnayake, Danni
- Keywords
- FACS, Muscle injury, Muscle progenitors, Muscle stem cells, Needle stab injury, Regeneration, Satellite cells, Skeletal muscle, Zebrafish larvae
- MeSH Terms
-
- Animals
- Biomarkers*
- Flow Cytometry*/methods
- Immunophenotyping
- Muscles/injuries*
- Myoblasts/cytology*
- Myoblasts/metabolism*
- Phenotype
- Regeneration
- Zebrafish
- PubMed
- 30367418 Full text @ Meth. Mol. Biol.
Citation
Ratnayake, D., Currie, P.D. (2019) Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury. Methods in molecular biology (Clifton, N.J.). 1889:245-254.
Abstract
This chapter describes a protocol for the isolation of larval zebrafish muscle stem/progenitor cells by fluorescence-activated cell sorting (FACS). This method has been successfully applied to isolate pax3a expressing cells 3 days following needle stab skeletal muscle injury. The cell sorting strategy described here can easily be adapted to any cell type at embryonic or larval stages. RNA extracted from the sorted cells can be used for subsequent downstream applications such as quantitative PCR (qPCR), microarrays, or next generation sequencing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping