PUBLICATION
FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos
- Authors
- Soh, G.H., Müller, P.
- ID
- ZDB-PUB-181017-13
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1863: 107-124 (Other)
- Registered Authors
- Müller, Patrick
- Keywords
- Developmental biology, Extracellular signaling molecules, FRAP, Fluorescence Recovery After Photobleaching, Morphogens, Zebrafish
- MeSH Terms
-
- Animals
- Biological Transport
- Diffusion
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/physiology*
- Fluorescence Recovery After Photobleaching/methods*
- Image Processing, Computer-Assisted/methods*
- Models, Biological
- Morphogenesis*
- Signal Transduction
- Software
- Zebrafish/embryology*
- Zebrafish/physiology*
- PubMed
- 30324594 Full text @ Meth. Mol. Biol.
Citation
Soh, G.H., Müller, P. (2018) FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos. Methods in molecular biology (Clifton, N.J.). 1863:107-124.
Abstract
Morphogens are signaling molecules that provide positional information to cells during development. They must move through embryonic tissues in order to coordinate patterning. The rate of a morphogen's movement through a tissue-its effective diffusivity-affects the morphogen's distribution and therefore influences patterning. Fluorescence recovery after photobleaching (FRAP) is a powerful method to measure the effective diffusion of molecules through cells and tissues, and has been successfully employed to examine morphogen mobility and gain important insights into embryogenesis. Here, we provide detailed protocols for FRAP assays in vitro and in living zebrafish embryos, and we explain how to analyze FRAP data using the open-source software PyFRAP to determine effective diffusion coefficients.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping