PUBLICATION

Fishing for understanding: Unlocking the zebrafish gene editor's toolbox

Authors
Simone, B.W., Martínez-Gálvez, G., WareJoncas, Z., Ekker, S.C.
ID
ZDB-PUB-180805-3
Date
2018
Source
Methods (San Diego, Calif.)   150: 3-10 (Other)
Registered Authors
Ekker, Stephen C.
Keywords
CRISPR, DNA repair, Genome editing, base editing, designer nuclease, zebrafish
MeSH Terms
  • Animals
  • CRISPR-Cas Systems/genetics
  • DNA Repair/genetics
  • Deoxyribonucleases/genetics*
  • Deoxyribonucleases/metabolism
  • Gene Editing/methods*
  • Genome/genetics
  • Protein Engineering
  • Zebrafish/genetics*
PubMed
30076892 Full text @ Methods
Abstract
The rapid growth of the field of gene editing can largely be attributed to the discovery and optimization of designer endonucleases. These include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regular interspersed short palindromic repeat (CRISPR) systems including Cas9, Cas12a, and structure-guided nucleases. Zebrafish (Danio rerio) have proven to be a powerful model system for genome engineering testing and applications due to their external development, high fecundity, and ease of housing. As the zebrafish gene editing toolkit continues to grow, it is becoming increasingly important to understand when and how to utilize which of these technologies for maximum efficacy in a particular project. While CRISPR-Cas9 has brought broad attention to the field of genome engineering in recent years, designer endonucleases have been utilized in genome engineering for more than two decades. This chapter provides a brief overview of designer endonuclease and other gene editing technologies in zebrafish as well as some of their known functional benefits and limitations depending on specific project goals. Finally, selected prospects for additional gene editing tools are presented, promising additional options for directed genomic programming of this versatile animal model system.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping