PUBLICATION

Developing and interpreting aqueous functional assays for comparative property-activity relationships of different nanoparticles

Authors
Kidd, J.M., Hanigan, D., Truong, L., Hristovski, K., Tanguay, R., Westerhoff, P.
ID
ZDB-PUB-180727-4
Date
2018
Source
The Science of the total environment   628-629: 1609-1616 (Journal)
Registered Authors
Tanguay, Robyn L.
Keywords
Exposure, Fate, Hazard, Nanoparticle, Water
MeSH Terms
none
PubMed
30045577 Full text @ Sci. Total Environ.
Abstract
It is difficult to relate intrinsic nanomaterial properties to their functional behavior in the environment. Unlike frameworks for dissolved organic chemicals, there are few frameworks comparing multiple and inter-related properties of engineered nanomaterials (ENMs) to their fate, exposure, and hazard in environmental systems. We developed and evaluated reproducibility and inter-correlation of 12 physical, chemical, and biological functional assays in water for eight different engineered nanomaterials (ENMs) and interpreted results using activity-profiling radar plots. The functional assays were highly reproducible when run in triplicate (average coefficient of variation [CV]=6.6%). Radar plots showed that each nanomaterial exhibited unique activity profiles. Reactivity assays showed dissolution or aggregation potential for some ENMs. Surprisingly, multi-walled carbon nanotubes (MWCNTs) exhibited movement in a magnetic field. We found high inter-correlations between cloud point extraction (CPE) and distribution to sewage sludge (R2=0.99), dissolution at pH8 and pH4.9 (R2=0.98), and dissolution at pH8 and zebrafish mortality at 24hpf (R2=0.94). Additionally, most ENMs tend to distribute out of water and into other phases (i.e., soil surfaces, surfactant micelles, and sewage sludge). The activity-profiling radar plots provide a framework and estimations of likely ENM disposition in the environment.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping