PUBLICATION

Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae

Authors
Huemer, K., Squirrell, J.M., Swader, R., Pelkey, K., LeBert, D.C., Huttenlocher, A., Eliceiri, K.W.
ID
ZDB-PUB-171121-11
Date
2017
Source
Journal of visualized experiments : JoVE   (128): (Journal)
Registered Authors
Huttenlocher, Anna
Keywords
none
MeSH Terms
  • Animals
  • Developmental Biology/methods*
  • Diagnostic Imaging/methods*
  • Disease Models, Animal
  • Larva
  • Zebrafish/embryology*
PubMed
29155730 Full text @ J. Vis. Exp.
Abstract
The zebrafish larva is an important model organism for both developmental biology and wound healing. Further, the zebrafish larva is a valuable system for live high-resolution microscopic imaging of dynamic biological phenomena in space and time with cellular resolution. However, the traditional method of agarose encapsulation for live imaging can impede larval development and tissue regrowth. Therefore, this manuscript describes the zWEDGI (zebrafish Wounding and Entrapment Device for Growth and Imaging), which was designed and fabricated as a functionally compartmentalized device to orient larvae for high-resolution microscopy while permitting caudal fin transection within the device and subsequent unrestrained tail development and re-growth. This device allows for wounding and long-term imaging while maintaining viability. Given that the zWEDGI mold is 3D printed, the customizability of its geometries make it easily modified for diverse zebrafish imaging applications. Furthermore, the zWEDGI offers numerous benefits, such as access to the larva during experimentation for wounding or for the application of reagents, paralleled orientation of multiple larvae for streamlined imaging, and reusability of the device.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping