PUBLICATION

PDGF controls contact inhibition of locomotion by regulating N-cadherin during neural crest migration

Authors
Bahm, I., Barriga, E.H., Frolov, A., Theveneau, E., Frankel, P., Mayor, R.
ID
ZDB-PUB-170521-2
Date
2017
Source
Development (Cambridge, England)   144(13): 2456-2468 (Journal)
Registered Authors
Mayor, Roberto
Keywords
EMT, N-cadherin, Neural crest, PDGF, contact inhibition of locomotion, migration
MeSH Terms
  • Animals
  • Cadherins/metabolism*
  • Cell Movement*/drug effects
  • Chemotaxis/drug effects
  • Contact Inhibition*/drug effects
  • Locomotion*/drug effects
  • Neural Crest/cytology*
  • Phosphatidylinositol 3-Kinases/metabolism
  • Platelet-Derived Growth Factor/metabolism*
  • Proto-Oncogene Proteins c-akt/metabolism
  • Receptor, Platelet-Derived Growth Factor alpha/metabolism
  • Signal Transduction/drug effects
  • Small Molecule Libraries/pharmacology
  • Xenopus laevis/metabolism*
PubMed
28526750 Full text @ Development
Abstract
A fundamental property of neural crest (NC) migration is Contact inhibition of locomotion (CIL), a process by which cells change their direction of migration upon cell contact. CIL has been proven to be essential for NC migration in amphibian and zebrafish by controlling cell polarity in a cell contact dependent manner. Cell contact during CIL requires the participation of the cell adhesion molecule N-cadherin, which starts to be expressed by NC cells as a consequence of the switch between E- and N-cadherins during epithelial to mesenchymal transition (EMT). However, the mechanism that controls the upregulation of N-cadherin remains unknown. Here we show that PDGFRα and its ligand PDGF-A are co-expressed in migrating cranial NC. Inhibition of PDGF-A/PDGFRα blocks NC migration by inhibiting N-cadherin and, consequently impairing CIL. Moreover, we find PI3K/AKT as a downstream effector of the PDGFRα cellular response during CIL. Our results lead us to propose PDGF-A/PDGFRα signalling as a tissue-autonomous regulator of CIL by controlling N-cadherin upregulation during EMT. Finally, we show that once NC have undergone EMT, the same PDGF-A/PDGFRα works as NC chemoattractant guiding their directional migration.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping