PUBLICATION
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
- Authors
- Welch, E.L., Eno, C.C., Nair, S., Lindeman, R.E., Pelegri, F.
- ID
- ZDB-PUB-170519-4
- Date
- 2017
- Source
- Journal of visualized experiments : JoVE (122): (Journal)
- Registered Authors
- Nair, Sreelaja, Pelegri, Francisco
- Keywords
- none
- MeSH Terms
-
- Animals
- Female
- Fertilization in Vitro
- In Vitro Oocyte Maturation Techniques*
- Oocytes/growth & development
- Oogenesis
- Zebrafish/embryology
- PubMed
- 28518112 Full text @ J. Vis. Exp.
Citation
Welch, E.L., Eno, C.C., Nair, S., Lindeman, R.E., Pelegri, F. (2017) Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish. Journal of visualized experiments : JoVE. (122).
Abstract
Cellular events that take place during the earliest stages of animal embryonic development are driven by maternally derived gene products deposited into the developing oocyte. Because these events rely on maternal products which typically act very soon after fertilization-that preexist inside the egg, standard approaches for expression and functional reduction involving the injection of reagents into the fertilized egg are typically ineffective. Instead, such manipulations must be performed during oogenesis, prior to or during the accumulation of maternal products. This article describes in detail a protocol for the in vitro maturation of immature zebrafish oocytes and their subsequent in vitro fertilization, yielding viable embryos that survive to adulthood. This method allows the functional manipulation of maternal products during oogenesis, such as the expression of products for phenotypic rescue and tagged construct visualization, as well as the reduction of gene function through reverse-genetics agents.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping